The porcine cerebellin gene family

Cerebellins (CBLN1–4), together with C1qTNF proteins, belong to the CBLN subfamily of C1q proteins. Cerebellin-1 (CBLN1) is active in synapse formation and functions at parallel fiber-Purkinje cell synapses. form tripartite complexes neurexins glutamate-receptor-related proteins GluD1 GluD2, playing a role as trans-synaptic cell-adhesion molecules that critically contribute both functioning brain development. In this study, I present molecular characterization four porcine genes. Experimental data silico analyses collectively describes gene structure, chromosomal localization, expression CBLN1-4. Two cDNAs encoding cerebellins CBLN1 CBLN3 were RT-PCR cloned sequenced. The nucleotide sequence clone contains an open reading frame 582 nucleotides encodes protein 193 amino acids. deduced acid was 99% identical mouse human CBLN1. putative signal 21 residues, two conserved cysteine domain. cDNA comprises 618 205 88% 94% CBLN3. terminal ends contain three possible N-linked glycosylation sites. genomic organization very similar those their counterparts. demonstrated transcripts are predominantly expressed cerebellum. sequences precerebellin genes submitted DDBJ/EMBL/GenBank under following accession numbers: (GenBank ID: FJ621565), EF577504), FJ621566), EF577505) CBLN4 FJ196070).